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王姿颖博士论文被Free Radical Biology & Medicine(IF:5.271)接受
作者:  来源:   发布时间:2013-08-16 08:02:49  浏览次数:

NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of

Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke

 

Ziying Wanga, Xinbing Weia, Kang Liua, Xiumei Zhanga, Fan Yanga, Hongyu Zhangb, Yeteng Hec, Tianfeng Zhua, Fengli Lia Weichen Shia, Yan Zhanga, Huiyan Xua, Jiang Liua, and Fan Yia*

 

aDepartment of Pharmacology, Shandong University School of Medicine, Jinan, China, 250012

bDepartment of Geriatrics, Qilu Hospital of Shandong University, Jinan, 250012, China.

cDepartment of Orthopedics, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China, 250014

 

Running title: NOX2-mediated complexin II signaling in ischemic stroke

 

Send Correspondence and

Reprint Requests to:  

Fan Yi, Ph.D
Professor

Department of Pharmacology

Shandong University School of Medicine

#44, Wenhua Xi Road,

Jinan, Shandong, 250012, P.R. China

Phone : 86-0531-88382616

Fax :   86-0531-88382616

E-mail: fanyi@sdu.edu.cn


Abstract

Although NADPH oxidase-mediated oxidative stress is considered as one of the major mechanisms triggering the pathogenic actions of ischemic stroke and very recent studies have indicated that NADPH oxidase is a major source of reactive oxygen species (ROS) production controlling glutamate release, how neuronal NADPH oxidase activation is coupled to glutamate release is not well understood. Therefore, in this study, we used an in vivo transient middle cerebral artery occlusion (MCAO) model and in vitro primary cell cultures to test whether complexins, the regulators of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion, are associated with NOX2-derived ROS and contribute to glutamate-mediated excitotoxicity in ischemic stroke. In this study, we first identified the upregulation of complexin II in the ischemic brain and evaluated the potential role in ischemic stroke showing that gene silencing of complexin II ameliorated cerebral injury as evidenced by reduced infarction volume, neurological deficit, neuron necrosis accompanied by decreased glutamate levels in consistent with the results from NOX2-/- mice with ischemic stroke. We further demonstrated complexin II expression was mediated by NOX2 in primary cultured neurons subjected to oxygen-glucose deprivation (OGD) and contributed to OGD-induced glutamate release and neuron necrosis via SNARE signaling. Taken together, these findings for the first time provide evidence that complexin II as a central target molecule that links NADPH oxidase-derived ROS to glutamate-mediated neuronal excitotoxicity in ischemic stroke.

 

 

 

 

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